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Sterilization of tissue‐culture medium by filtration on a large laboratory scale
Author(s) -
Telling R. C.,
Stone C. J.,
Maskell M. A.
Publication year - 1966
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260080113
Subject(s) - sterilization (economics) , filtration (mathematics) , tissue culture , plant tissue , chromatography , chemistry , pulp and paper industry , botany , biology , engineering , in vitro , biochemistry , mathematics , business , statistics , finance , exchange rate , foreign exchange market
The design and operation of a permanently‐piped plant for sterilizing 10–200 l. of tissue‐culture media is described. The plant is sterilized in situ by steam injection, the filtration rate is 2–3 l./min., and the turn‐around time for volumes of 100 l. is about 3 hr.