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Production of Bacillus cereus exopenicillinase on a pilot‐plant scale
Author(s) -
Miller G.,
Bach G.,
Markus Z.
Publication year - 1965
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260070407
Subject(s) - bacillus cereus , fermentation , cereus , food science , chemistry , yield (engineering) , chromatography , gram , pilot plant , bacteria , biology , organic chemistry , materials science , genetics , metallurgy
A substrain of Bacillus cereus 569/H produced above 10,000 units of penicillinase/ ml. when grown in a pilot‐plant fermentor using a medium containing Casamino acids techn. (Difco) or N‐Z‐Amine type B (Sheffield), and salts. Simplified purification and concentration procedures give an overall yield of 50‐65% enzyme. The freeze‐dried enzyme preparation had a good storage stability in vacuumsealed ampules kept at 4, 30, and 37°C. In vials containing air in the head space, partial inactivation occurred in two months at 30 and 37°C. The freezedried preparation showed satisfactory performance in the production of yoghurt fermented milk.

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