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A practical method for partial purification of the organism of psittacosis grown in chicken embryo yolk sac
Author(s) -
Shirey William N.,
Dorsey Warren G.,
Patrick William C.
Publication year - 1965
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260070402
Subject(s) - psittacosis , yolk , centrifugation , chromatography , biology , antigenicity , biochemistry , chemistry , embryo , food science , virology , antigen , microbiology and biotechnology , genetics
A procedure described for the partial purification of suspensions of the organisms of psittacosis utilizes tryptic digestion, calcium chloridedextran sulfate precipitation, and centrifugation through a sucrose barrier. More than 40% of the organisms in the original suspension are recovered in 80% of the original volume, with approximately 95% of the lipids and 85% of the proteins removed. This method is suggested as a preliminary purification procedure in laboratories desiring relatively large amounts of purified agent for studies on metabolism, physical properties, or antigenicity of the organism. Significant aspects of the investigations leading to the selection of the procedures are included.