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Extraction and separation of nucleotides from Escherichia coli
Author(s) -
Lilly M. D.
Publication year - 1965
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260070303
Subject(s) - nucleotide , chromatography , chemistry , elution , lithium chloride , hydrochloric acid , centrifugation , extraction (chemistry) , yield (engineering) , ion exchange , chloride , adsorption , escherichia coli , biochemistry , organic chemistry , ion , materials science , metallurgy , gene
A laboratory process for the isolation of acid soluble nucleotides from bacteria has been scaled‐up. Escherichia coli was grown in 220‐l. batches, harvested by centrifugation, and extracted with cold trichloracetic acid. The nucleotides in the extract were separated by adsorption onto a Dowex‐1 ion exchange resin column followed by elution with dilute hydrochloric acid and lithium chloride. After concentration of the effluent, the lithium chloride was removed by extraction with organic solvents. The overall yield of nucleotides was 75–80%. This elution system has also been used for the separation of nucleotides from bakers' yeast extracts.