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Sterilization of microbiological media with β‐propiolactone
Author(s) -
Holme Tord
Publication year - 1965
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260070122
Subject(s) - sterilization (economics) , titration , chemistry , hydrolysis , chromatography , pulp and paper industry , waste management , organic chemistry , business , engineering , finance , exchange rate , foreign exchange market
A procedure for sterilization of microbiological media with β‐propiolactone has been developed. Special attention was paid to the maintenance of mild conditions to enable the treatment of media sensitive to high temperature or low pH. The maximum temperature allowed was 40°C. and automatic neutralization of acid produced during hydrolysis was effected by the use of titration unit. The apparatus could be used for several successive sterilization cycles without disconnection.