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Studies on the purification of vaccinia virus
Author(s) -
Orlando M. D.,
Riley J. M.,
Patrick W. C.
Publication year - 1964
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260060307
Subject(s) - infectivity , vaccinia , embryonated , titer , virus , centrifugation , chromatography , differential centrifugation , virology , chemistry , extraction (chemistry) , biology , biochemistry , gene , recombinant dna
Various methods for the removal of extraneous matter from vaccinia virus suspensions are described and compared. Of ten procedures tested, differential centrifugation and Freon extraction were the most successful. Both the partially purified suspensions and the dried products prepared from them had high virus titers in embryonated eggs and high infectivity for mice. Although both processes resulted in products with the same titers, the differentially centrifuged product was approximately tenfold more purified as shown by the percentage solids of the suspensions before freeze drying.