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An apparatus for continuous electrophoresis on a preparative scale
Author(s) -
Blakebrough N.,
Brookes R.
Publication year - 1964
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260060209
Subject(s) - thin layer , electrophoresis , buffer (optical fiber) , layer (electronics) , chromatography , scale (ratio) , materials science , chemistry , computer science , nanotechnology , physics , telecommunications , quantum mechanics
A method of performing electrophoretic separations in a thin horizontal layer of continuously flowing buffer has been developed. The theoretical considerations that make possible the use of a thin layer for stabilization against convection effects, without the assistance of a packing material, are discussed. The apparatus is described together with an example of its performance, and a comparison with that of other workers.