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A method of automatic pH control of a bicarbonate‐CO 2 buffer system for the submerged culture of hamster kidney cells
Author(s) -
Telling R. C.,
Stone C. J.
Publication year - 1964
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260060204
Subject(s) - bicarbonate , baby hamster kidney cell , chromatography , hamster , sodium bicarbonate , buffer (optical fiber) , chemistry , clone (java method) , suspension culture , suspension (topology) , cell culture , biology , biochemistry , cell , computer science , mathematics , microbiology and biotechnology , pure mathematics , dna , telecommunications , genetics , organic chemistry , homotopy
The equipment and operational techniques are described which were found suitable to control pH in the range 6.8–7.8 pH, within ±0.03 pH units of the desired value, at cell concentrations up to a maximum of 2.5 × 10 6 /ml. The results of batch growth of a suspension strain of the BHK cell (clone 13) under conditions of controlled pH are given and the significance of these results is discussed.