Premium
Uniform inoculation of nutrient surfaces
Author(s) -
Clark D. S.
Publication year - 1963
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260050208
Subject(s) - inoculation , nozzle , suspension (topology) , petri dish , nutrient agar , nutrient , spray nozzle , agar , zoology , materials science , chemistry , biology , mathematics , horticulture , microbiology and biotechnology , bacteria , ecology , physics , genetics , homotopy , pure mathematics , thermodynamics
A method of inoculating nutrient surfaces uniformly with bacterial cells has been developed. The method is based on controlled exposure of samples in a humidified chamber to a spray produced from a bacterial suspension. Factors affecting uniformity of inoculation (ionic strength of suspension, force of spray, distance between spray nozzle and sample, and exposure time) were studied in detail. Under optimum condition using agar medium in Petri plates, differences between total counts for six plates inoculated simultaneously, or between counts for individual 2 cm. 2 areas taken at any location on any of the plates, were not statistically significant at the 5% level. The coefficient of variability for counts on 2 cm. 2 areas renged from 8 to 12% between tests of 216 counts each.