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Fractionation of cell constituents of Bacillus megaterium in a polymer two‐phase system
Author(s) -
v. Hofsten Bengt,
Baird G. David
Publication year - 1962
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260040406
Subject(s) - bacillus megaterium , fractionation , chromatography , spore , polymer , dextran , chemistry , phase (matter) , protoplast , aqueous solution , biochemistry , biology , botany , organic chemistry , bacteria , genetics
The distribution of various cell constituents of Bacillus megaterium has been studied in an aqueous two‐phase system containing the two polymers dextran and polythylene glycol. The difference in behaviour between cell walls, lipid granules and soluble cell material are such that a considerable fractionation of a suspension of disrupted cells may be obtained after a few counter‐current distributions. A mixture of whole cells and protoplasts can also be fractionated in this phase system. If sodium chloride is added to the phase system, vegetative cells and spores can be separated from each other.