Premium
Continuous chemical sterilization of microbiological media with β‐propiolactone
Author(s) -
Toplin Irving
Publication year - 1962
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260040309
Subject(s) - sterilization (economics) , chemistry , food science , chromatography , pulp and paper industry , business , engineering , finance , exchange rate , foreign exchange market
A laboratory bench set‐up for the continuous chemical sterilization of microbiological media using β‐propiolactone was developed. The apparatus permits the sterilization of up to 4 liters/hr. of liquid media. The parameters of hydrolyzing time and temperature necessary to reduce the β‐propiolactone to nontoxic levels were determined using a E. coli ‐nutrient broth system. The sterilization technique was applied successfully to a variety of media, and its potential usefulness on an industrial scale is discussed.