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Mass cultivation of algae in pure heavy water
Author(s) -
Daboll Homer F.,
Crespi Henry L.,
Katz Joseph J.
Publication year - 1962
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260040304
Subject(s) - chlorella pyrenoidosa , algae , chlorella vulgaris , green algae , scenedesmus obliquus , chlorella , botany , cyanobacteria , blue green algae , chlorophyceae , chlorophyta , biology , scenedesmus , dry weight , chemistry , bacteria , genetics
Strains of the green algae Scenedesmus obliquus, Chlorella vulgaris , and C. pyrenoidosa 7‐11‐05, and of the blue‐green algae Fremyella diplosiphon, Plectonema calothricoides, Phormidium luridum , and Synechococcus lividus have been mass cultured autotrophically in essentially pure D 2 O. Closed Lucite rocking‐box units containing 5 liters of culture media can produce 1 to 2 g. (dry weight) per day of fully deuteriated algae or of fully deuteriated, fully 15 N‐substituted algae. Racks for six such culture units are readily constructed from commerically available parts. Operation has been routine and essentially trouble‐free. The algae produced have proved to be an excellent source of completely isotopically substituted metabolites.