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A modified adsorption method for determining percentage of dead yeast cells
Author(s) -
Vairo Marina L. R.
Publication year - 1962
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260040215
Subject(s) - yeast , adsorption , methylene blue , freundlich equation , aqueous solution , chemistry , chromatography , biochemistry , organic chemistry , photocatalysis , catalysis
The adsorption of methylene blue from different aqueous solutions by dead yeast cells follows Freundlich's law when the percentage of dead cells varies from 0 to 100. When the Fink and Kühles methylene blue was used, there was observed a linear relation between the percentage of dead cells and the dye concentration at the equilibrium. The experimental errors were measured. A simpler and more precise colorimetric method for determining the percentage of dead yeast cells is deseribed.