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Fully automatized high‐throughput enzyme library screening using a robotic platform
Author(s) -
Dörr Mark,
Fibinger Michael P.C.,
Last Daniel,
Schmidt Sandy,
SantosAberturas Javier,
Böttcher Dominique,
Hummel Anke,
Vickers Clare,
Voss Moritz,
Bornscheuer Uwe T.
Publication year - 2016
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25925
Subject(s) - high throughput screening , protein engineering , biocatalysis , microtiter plate , throughput , enzyme , computational biology , computer science , chemistry , biochemical engineering , biochemistry , biology , chromatography , engineering , telecommunications , ionic liquid , wireless , catalysis
A fully automatized robotic platform has been established to facilitate high‐throughput screening for protein engineering purposes. This platform enables proper monitoring and control of growth conditions in the microtiter plate format to ensure precise enzyme production for the interrogation of enzyme mutant libraries, protein stability tests and multiple assay screenings. The performance of this system has been exemplified for four enzyme classes important for biocatalysis such as Baeyer–Villiger monooxygenase, transaminase, dehalogenase and acylase in the high‐throughput screening of various mutant libraries. This allowed the identification of novel enzyme variants in a sophisticated and highly reliable manner. Furthermore, the detailed optimization protocols should enable other researchers to adapt and improve their methods. Biotechnol. Bioeng. 2016;113: 1421–1432. © 2016 Wiley Periodicals, Inc.