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Lactic acid production from cellobiose and xylose by engineered Saccharomyces cerevisiae
Author(s) -
Turner Timothy L.,
Zhang GuoChang,
Oh Eun Joong,
Subramaniam Vijay,
Adiputra Andrew,
Subramaniam Vimal,
Skory Christopher D.,
Jang Ji Yeon,
Yu Byung Jo,
Park In,
Jin YongSu
Publication year - 2016
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25875
Subject(s) - cellobiose , xylose , biochemistry , chemistry , fermentation , lactic acid , lignocellulosic biomass , metabolic engineering , lactic acid fermentation , food science , cellulose , biology , cellulase , bacteria , enzyme , genetics
Efficient and rapid production of value‐added chemicals from lignocellulosic biomass is an important step toward a sustainable society. Lactic acid, used for synthesizing the bioplastic polylactide, has been produced by microbial fermentation using primarily glucose. Lignocellulosic hydrolysates contain high concentrations of cellobiose and xylose. Here, we constructed a recombinant Saccharomyces cerevisiae strain capable of fermenting cellobiose and xylose into lactic acid. Specifically, genes ( cdt‐1 , gh1‐1 , XYL1 , XYL2 , XYL3 , and ldhA ) coding for cellobiose transporter, β‐glucosidase, xylose reductase, xylitol dehydrogenase, xylulokinase, and lactate dehydrogenase were integrated into the S. cerevisiae chromosomes. The resulting strain produced lactic acid from cellobiose or xylose with high yields. When fermenting a cellulosic sugar mixture containing 10 g/L glucose, 40 g/L xylose, and 80 g/L cellobiose, the engineered strain produced 83 g/L of lactic acid with a yield of 0.66 g lactic acid/g sugar (66% theoretical maximum). This study demonstrates initial steps toward the feasibility of sustainable production of lactic acid from lignocellulosic sugars by engineered yeast. Biotechnol. Bioeng. 2016;113: 1075–1083. © 2015 Wiley Periodicals, Inc.