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Continuous downstream processing for high value biological products: A Review
Author(s) -
Zydney Andrew L.
Publication year - 2016
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25695
Subject(s) - bioprocess , downstream processing , process engineering , downstream (manufacturing) , continuous production , biochemical engineering , process analytical technology , bioreactor , unit operation , computer science , product (mathematics) , process (computing) , process development , manufacturing engineering , environmental science , engineering , chemistry , operations management , mathematics , chromatography , geometry , organic chemistry , chemical engineering , environmental engineering , operating system
There is growing interest in the possibility of developing truly continuous processes for the large‐scale production of high value biological products. Continuous processing has the potential to provide significant reductions in cost and facility size while improving product quality and facilitating the design of flexible multi‐product manufacturing facilities. This paper reviews the current state‐of‐the‐art in separations technology suitable for continuous downstream bioprocessing, focusing on unit operations that would be most appropriate for the production of secreted proteins like monoclonal antibodies. This includes cell separation/recycle from the perfusion bioreactor, initial product recovery (capture), product purification (polishing), and formulation. Of particular importance are the available options, and alternatives, for continuous chromatographic separations. Although there are still significant challenges in developing integrated continuous bioprocesses, recent technological advances have provided process developers with a number of attractive options for development of truly continuous bioprocessing operations. Biotechnol. Bioeng. 2016;113: 465–475. © 2015 Wiley Periodicals, Inc.