High‐rate volatile fatty acid (VFA) production by a granular sludge process at low pH

Volatile fatty acids (VFA) are proposed platform molecules for the production of basic chemicals and polymers from organic waste streams. In this study we developed a granular sludge process to produce VFA at high rate, yield and purity while minimizing potential operational costs. A lab‐scale anaerobic sequencing batch reactor (ASBR) was fed with 10 g l −1 glucose as model substrate. Inclusion of a short (2 min) settling phase before effluent discharge enabled effective granulation and very high volumetric conversion rates of 150–300 gCOD l −1  d −1 were observed during glucose conversion. The product spectrum remained similar at the tested pH range with acetate and butyrate as the main products, and a total VFA yield of 60–70% on chemical oxygen demand (COD) basis. The requirement for base addition for pH regulation could be reduced from 1.1 to 0.6 mol OH − (mol glucose) −1 by lowering the pH from 5.5 to 4.5. Solids concentrations in the effluent were 0.6 ± 0.3 g l −1 but could be reduced to 0.02 ± 0.01 g l −1 by introduction of an additional settling period of 5 min. The efficient production of VFA at low pH with a virtually solid‐free effluent increases the economic feasibility of waste‐based chemicals and polymer production. Biotechnol. Bioeng. 2015;112: 2248–2255. © 2015 Wiley Periodicals, Inc.