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Optimization of surface‐immobilized extracellular matrices for the proliferation of neural progenitor cells derived from induced pluripotent stem cells
Author(s) -
Komura Takashi,
Kato Koichi,
Konagaya Shuhei,
NakajiHirabayashi Tadashi,
Iwata Hiroo
Publication year - 2015
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25636
Subject(s) - laminin , progenitor cell , neural stem cell , induced pluripotent stem cell , matrigel , microbiology and biotechnology , neurosphere , stem cell , extracellular matrix , transplantation , progenitor , biology , chemistry , endothelial stem cell , cell , biochemistry , adult stem cell , embryonic stem cell , in vitro , medicine , surgery , gene
Neural progenitor cells derived from induced pluripotent stem cells have been considered as a potential source for cell‐transplantation therapy of central nervous disorders. However, efficient methods to expand neural progenitor cells are further required for their clinical applications. In this study, a protein array was fabricated with nine extracellular matrices and used to screen substrates suitable for the expansion of neural progenitor cells derived from mouse induced pluripotent stem cells. The results showed that neural progenitor cells efficiently proliferated on substrates with immobilized laminin‐1, laminin‐5, or Matrigel. Based on this result, further attempts were made to develop clinically compliant substrates with immobilized polypeptides that mimic laminin‐1, one of the most effective extracellular matrices as identified in the array‐based screening. We used here recombinant DNA technology to prepare polypeptide containing the globular domain 3 of laminin‐1 and immobilized it onto glass‐based substrates. Our results showed that neural progenitor cells selectively proliferated on substrate with the immobilized polypeptide while maintaining their differentiated state. Biotechnol. Bioeng. 2015;112: 2388–2396. © 2015 Wiley Periodicals, Inc.