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Metabolic control of respiratory levels in coenzyme Q biosynthesis‐deficient Escherichia coli strains leading to fine‐tune aerobic lactate fermentation
Author(s) -
Wu Hui,
Bennett George N.,
San KaYiu
Publication year - 2015
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25585
Subject(s) - biosynthesis , escherichia coli , mutant , biochemistry , cofactor , metabolic pathway , fermentation , metabolic engineering , yield (engineering) , biology , respiratory chain , chemistry , metabolism , enzyme , gene , materials science , metallurgy
A novel strategy to finely control the electron transfer chain (ETC) activity of Escherichia coli was established. In this study, the fine‐tuning of the ubiquinone biosynthesis pathway was applied to further controlling ETC function in coenzyme Q8 biosynthesis‐deficient E. coli strains, HW108 and HW109, which contain mutations in ubiE and ubiG , respectively. A competing pathway on the intermediate substrates of the Q8 synthesis pathway, catalyzed by diphosphate:4‐hydroxybenzoate geranyltransferase (PGT‐1) of Lithospermum erythrorhizon , was introduced into these mutant strains. A nearly theoretical yield of lactate production can be achieved under fully aerobic conditions via an in vivo, genetically fine‐tunable means to further control the activity of the ETC of the Q8 biosynthesis‐deficient E. coli strains. Biotechnol. Bioeng. 2015;112: 1720–1726. © 2015 Wiley Periodicals, Inc.