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Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel
Author(s) -
Xu Yufan,
Wang Xiaohong
Publication year - 2015
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25579
Subject(s) - microfluidics , gelatin , 3d printed , biocompatible material , fibrin , spheroid , regenerative medicine , nanotechnology , tissue engineering , materials science , stem cell , biomedical engineering , chemistry , cell culture , microbiology and biotechnology , engineering , biology , biochemistry , immunology , genetics
ABSTRACT Three‐dimensional (3D) cell manipulation is available with the integration of microfluidic technology and rapid prototyping techniques. High‐Fidelity (Hi‐Fi) constructs hold enormous therapeutic potential for organ manufacturing and regenerative medicine. In the present paper we introduced a quasi‐three‐dimensional (Q3D) model with parallel biocompatible alginate/gelatin/fibrin hurdles. The behaviors of fluids and cells along the microfluidic channels with various widths were studied. Cells inside the newly designed microfluidic channels attached and grew well. Morphological changes of adipose‐derived stem cells (ADSCs) in both two‐dimensional (2D) and 3D milieu were found on the printed constructs. Endothelialization occurred with the co‐cultures of ADSCs and hepatocytes. This study provides insights into the interactions among fluids, cells and biomaterials, the behaviors of fluids and cells along the microfluidic channels, and the applications of Q3D techniques. Biotechnol. Bioeng. 2015;112: 1683–1695. © 2015 Wiley Periodicals, Inc.