Premium
Non‐destructive label‐free continuous monitoring of in vitro chondrogenesis via electrical conductivity and its anisotropy
Author(s) -
Oh Tong In,
Kim Changhwan,
Karki Bishal,
Son Youngsook,
Lee EunAh,
Woo Eung Je
Publication year - 2015
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25350
Subject(s) - anisotropy , cartilage , chondrogenesis , conductivity , biomedical engineering , materials science , electrical resistivity and conductivity , glycosaminoglycan , electrode , tissue engineering , in vitro , tissue culture , analytical chemistry (journal) , chemistry , anatomy , chromatography , medicine , biochemistry , electrical engineering , optics , physics , engineering
Non‐destructive label‐free continuous monitoring of in vitro tissue culture is an unmet demand in tissue engineering. Noting that different compositions of cartilage lead to different electrical tissue properties, we propose a new method to measure the electrical conductivity and its anisotropy during in vitro chondrogenesis. We used a conductivity tensor probe with 17 electrodes and a bio‐impedance spectroscopy (BIS) device to measure the conductivity values and the anisotropy ratios at the bottom and top surfaces of the tissue samples during the culture period of 6 weeks. Clearly distinguishing glycosaminoglycans (GAGs), collagen, and also various mixtures of them, the measured conductivity value and the estimated tissue anisotropy provide diagnostic information of the depth‐dependent tissue structure and compositions. Continuously monitoring the individual tissue during the entire chondrogenesis process without any adverse effect, the proposed method may significantly increase the productivity of cartilage tissue engineering. Biotechnol. Bioeng. 2015;112: 422–427. © 2014 Wiley Periodicals, Inc.