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Induction of ganoderic acid biosynthesis by Mn 2+ in static liquid cultivation of Ganoderma lucidum
Author(s) -
Xu YiNing,
Xia XiaoXia,
Zhong JianJiang
Publication year - 2014
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25288
Subject(s) - calcineurin , intracellular , chemistry , biosynthesis , metabolism , biochemistry , ganoderma , microbiology and biotechnology , ganoderma lucidum , signal transduction , biology , gene , food science , transplantation , medicine , surgery
Metal ions affect cell physiology and metabolism significantly, but the role of Mn 2+ in the secondary metabolism of mushrooms is yet unclear. In static liquid cultivation of Ganoderma lucidum for producing antitumor ganoderic acids (GAs), the Mn 2+ addition was performed. Addition of 10 mM Mn 2+ at the start of the static liquid cultivation resulted in 2.2‐fold improvement of total GAs production. The expression levels of GA biosynthetic and Ca 2+ sensors' genes were up‐regulated with Mn 2+ induction while down‐regulated by adding cyclosporin A (calcineurin inhibitor), suggesting that higher GA production might result from calcineurin signal regulation. Intracellular Ca 2+ imaging and calcineurin inhibitor study revealed that addition of Mn 2+ led to Ca 2+ influx from medium to the cells to trigger calcineurin signals. Mn 2+ addition was therefore an efficient induction strategy for improving GAs production, whose regulation mechanism was via calcineurin signaling transduction. Biotechnol. Bioeng. 2014;111: 2358–2365. © 2014 Wiley Periodicals, Inc.