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Bacterial community structure and activity of sulfate reducing bacteria in a membrane aerated biofilm analyzed by microsensor and molecular techniques
Author(s) -
Liu Hong,
Tan Shuying,
Sheng Zhiya,
Liu Yang,
Yu Tong
Publication year - 2014
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25277
Subject(s) - biofilm , bacteria , anoxic waters , temperature gradient gel electrophoresis , chemistry , aeration , microbiology and biotechnology , gel electrophoresis , sulfate reducing bacteria , sulfate , microaerophile , chromatography , membrane , environmental chemistry , biochemistry , biology , 16s ribosomal rna , gene , organic chemistry , genetics
The activities and vertical spatial distribution of sulfate reducing bacteria (SRB) in an oxygen (O 2 )‐based membrane aerated biofilm (MAB) were investigated using microsensor (O 2 and H 2 S) measurements and molecular techniques (polymerase chain reaction‐denaturing gradient gel electrophoresis [PCR‐DGGE] and fluorescence in situ hybridization [FISH]). The O 2 concentration profile revealed that O 2 penetrated from the bottom (substratum) of the gas permeable membrane, and was gradually consumed within the biofilm until it was completely depleted near the biofilm/bulk liquid interface, indicating oxic and anoxic zone in the MAB. The H 2 S concentration profile showed that H 2 S production was found in the upper 285 µm of the biofilm, indicating a high activity of SRB in this region. The results from DGGE of the PCR‐amplified dissimilatory sulfite reductase subunit B (dsrB) gene and FISH showed an uneven spatial distribution of SRB. The maximum SRB biomass was located in the upper biofilm. The information from the molecular analysis can be supplemented with that from microsensor measurements to better understand the microbial community and activity of SRB in the MAB. Biotechnol. Bioeng. 2014;111: 2155–2162. © 2014 Wiley Periodicals, Inc.

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