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Lipase‐catalyzed process for biodiesel production: Protein engineering and lipase production
Author(s) -
Hwang Hyun Tae,
Qi Feng,
Yuan Chongli,
Zhao Xuebing,
Ramkrishna Doraiswami,
Liu Dehua,
Varma Arvind
Publication year - 2014
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25162
Subject(s) - lipase , biodiesel , transesterification , biodiesel production , biochemical engineering , production (economics) , microbiology and biotechnology , chemistry , environmental science , catalysis , engineering , organic chemistry , biology , enzyme , economics , macroeconomics
Biodiesel is an environment‐friendly and renewable fuel produced by transesterification of various feedstocks. Although the lipase‐catalyzed biodiesel production has many advantages over the conventional alkali catalyzed process, its industrial applications have been limited by high‐cost and low‐stability of lipase enzymes. This review provides a general overview of the recent advances in lipase engineering, including both protein modification and production. Recent advances in biotechnology such as in protein engineering, recombinant methods and metabolic engineering have been employed but are yet to impact lipase engineering for cost‐effective production of biodiesel. A summary of the current challenges and perspectives for potential solutions are also provided. Biotechnol. Bioeng. 2014;111: 639–653. © 2013 Wiley Periodicals, Inc.