z-logo
Premium
Transfer printing of transfected cell microarrays from poly(ethylene glycol)‐oleyl surfaces onto biological hydrogels
Author(s) -
Yamaguchi Satoshi,
Komiya Senori,
Matsunuma Erika,
Yamahira Shinya,
Kihara Takanori,
Miyake Jun,
Nagamune Teruyuki
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.25010
Subject(s) - transfection , self healing hydrogels , ethylene glycol , lipofectamine , extracellular matrix , chemistry , cell adhesion , cell , micropatterning , tissue engineering , materials science , microbiology and biotechnology , biophysics , nanotechnology , biomedical engineering , biology , biochemistry , gene , polymer chemistry , organic chemistry , vector (molecular biology) , medicine , recombinant dna
We have developed a novel technique for constructing microarrays of transfected mammalian cells on or in extracellular matrix (ECM) hydrogels by transfer printing from patterned poly(ethylene glycol) (PEG)‐oleyl surfaces. A mixed solution of small interfering RNA (siRNA) and a transfection reagent was spotted on PEG‐oleyl‐coated glass slides using an ink‐jet printer, and the cells were then transiently immobilized on the patterned transfection mixtures. After overlaying an ECM hydrogel sheet onto the immobilized cells, the cells sandwiched between the glass slide and the hydrogel sheet were incubated at 37°C for simultaneous transfection of siRNA into cells and adhesion of cells to the hydrogel sheet. Transfer of the adhered, transfected cells was completed by peeling off the hydrogel sheet. The knockdown of a model gene in the transferred cell microarray by the transfected siRNA was successfully confirmed. Transfected cell microarrays were also embedded within three‐dimensional ECM hydrogels. In the three‐dimensional hydrogel, the inhibition effect of siRNA on cancer cell invasion was evaluated by quantifying the size of cell clusters on the microarrays. These results indicate that transfection of cell microarrays on or in a biological matrix is a promising technique for high‐throughput screening of disease‐related genes by direct observation of cellular phenomena in a physiologically relevant environment. Biotechnol. Bioeng. 2013;110: 3269–3274. © 2013 Wiley Periodicals, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here