Premium
Prediction of inclusion body solubilization from shaken to stirred reactors
Author(s) -
Walther Cornelia,
Mayer Sabrina,
Trefilov Alexandru,
Sekot Gerhard,
Hahn Rainer,
Jungbauer Alois,
Dürauer Astrid
Publication year - 2014
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24998
Subject(s) - dimensionless quantity , solubilization , mixing (physics) , continuous stirred tank reactor , chemistry , kinetics , chromatography , yield (engineering) , thermodynamics , chemical engineering , biochemistry , physics , engineering , quantum mechanics
Inclusion bodies (IBs) were solubilized in a µ ‐scale system using shaking microtiter plates or a stirred tank reactor in a laboratory setting. Characteristic dimensionless numbers for mixing, the Phase number Ph and Reynolds number Re did not correlate with the kinetics and equilibrium of protein solubilization. The solubilization kinetics was independent of the mixing system, stirring or shaking rate, shaking diameter, and energy input. Good agreement was observed between the solubilization kinetics and yield on the µ ‐scale and laboratory setting. We show that the IB solubilization process is controlled predominantly by pore diffusion. Thus, for the process it is sufficient to keep the IBs homogeneously suspended, and additional power input will not improve the process. The high‐throughput system developed on the µ ‐scale can predict solubilization in stirred reactors up to a factor of 500 and can therefore be used to determine optimal solubilization conditions on laboratory and industrial scale. Biotechnol. Bioeng. 2014;111: 84–94. © 2013 Wiley Periodicals, Inc.