Premium
Consolidated bioprocessing of highly concentrated jerusalem artichoke tubers for simultaneous saccharification and ethanol fermentation
Author(s) -
Guo Lihao,
Zhang Jian,
Hu Fengxian,
Dy Ryu Dewey,
Bao Jie
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24929
Subject(s) - bioprocess , jerusalem artichoke , bioreactor , inulin , fermentation , biofuel , chemistry , ethanol fuel , inulinase , food science , biomass (ecology) , raw material , pulp and paper industry , hydrolysis , ethanol fermentation , microbiology and biotechnology , agronomy , biochemistry , biology , chemical engineering , engineering , organic chemistry
Consolidated bioprocessing (CBP) of Jerusalem artichoke tuber (Jat) for ethanol production is one of the most promising options for an alternate biofuel technology development. The technical barriers include the weak saccharolytic enzyme (inulinase) activity of the fermentation strain, and the well mixing of the high viscous fermentation slurry at the highly concentrated Jat loading. In this study, Saccharomyces cerevisiae DQ1 was found to produce relatively large amount of inulinase for hydrolysis of inulin in Jat, and the helical ribbon stirring bioreactor used provided well mixing performance under the high Jat loading. Even a highly concentrated Jat loading up to 35% (w/w) in the helical ribbon bioreactor for CBP was allowed. The results obtained from this study have demonstrated a feasibility of developing a CBP process technology in the helical ribbon bioreactor for ethanol production at a high yield 128.7 g/L and the theoretical yield 73.5%, respectively. This level of ethanol yield from Jat is relatively higher than others reported so far. The results of this study could provide a practical CBP process technology in the helical ribbon bioreactor for economically sustainable alternate biofuel production using highly concentrated inulin containing biomass feedstock such as Jat, at least 35%. Biotechnol. Bioeng. 2013;110: 2606–2615. © 2013 Wiley Periodicals, Inc.