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Candida bombicola as a platform organism for the production of tailor‐made biomolecules
Author(s) -
Roelants Sophie L.K.W.,
Saerens Karen M.J.,
Derycke Thibaut,
Li Bing,
Lin YaoCheng,
Van de Peer Yves,
De Maeseneire Sofie L.,
Van Bogaert Inge N.A.,
Soetaert Wim
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24895
Subject(s) - yeast , biomolecule , organism , yield (engineering) , biology , computational biology , bioplastic , model organism , synthetic biology , biochemical engineering , microbiology and biotechnology , chemistry , biochemistry , gene , genetics , materials science , engineering , ecology , metallurgy
The yeast Candida bombicola is capable of producing high amounts (400 g/L) of the biosurfactant sophorolipids. The genetic makeup of this industrially important yeast has recently been uncovered and molecular manipulation techniques have been developed. Hence, all tools for the development of new bioprocesses with C. bombicola are now available. As a proof of concept, the production of two totally different molecules was aimed for: the bioplastic polyhydroxyalkanoate (PHA) and a new‐to‐nature cellobioselipid‐biosurfactant. Integration of the new functionalities at genomic loci necessary for sophorolipid production safeguards the new biomolecules from sophorolipid contamination, while taking advantage of the regulation of the sophorolipid gene cluster. A maximum yield of 2.0% wt/dwt PHA was obtained; furthermore, this is the first time cellobioselipid synthesis by a non‐natural producer is reported. We here provided proof of concept that C. bombicola can be transformed into a platform organism for the production of tailor‐made biomolecules. Biotechnol. Bioeng. 2013; 110:2494–2503. © 2013 Wiley Periodicals, Inc.