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Transcription activator‐like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing
Author(s) -
Sun Ning,
Zhao Huimin
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24890
Subject(s) - transcription activator like effector nuclease , genome editing , effector , biology , computational biology , dna , genome , transcription (linguistics) , modular design , crispr , genome engineering , genetics , computer science , gene , microbiology and biotechnology , programming language , linguistics , philosophy
Transcription activator‐like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site‐specific chromosomal double‐strand breaks introduced by TALENs significantly increase the efficiency of genomic modification. The modular nature of the TALE central repeat domains enables researchers to tailor DNA recognition specificity with ease and target essentially any desired DNA sequence. Here, we comprehensively review the development of TALEN technology in terms of scaffold optimization, DNA recognition, and repeat array assembly. In addition, we provide some perspectives on the future development of this technology. Biotechnol. Bioeng. 2013; 110: 1811–1821. © 2013 Wiley Periodicals, Inc.