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Reducing infectivity of HIV upon exposure to surfaces coated with N , N ‐dodecyl, methyl‐polyethylenimine
Author(s) -
Gerrard Stephen E.,
Larson Alyssa M.,
Klibanov Alexander M.,
Slater Nigel K.H.,
Hanson Carl V.,
Abrams Barbara F.,
Morris Mary K.
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24867
Subject(s) - polyethylenimine , infectivity , chemistry , titer , rna , polyethylene , biophysics , virus , chromatography , virology , microbiology and biotechnology , biochemistry , biology , organic chemistry , gene , transfection
Abstract The infectivity of high‐titer, cell‐free HIV in culture media and human milk is rapidly reduced upon exposure to polyethylene slides painted with the linear hydrophobic polycation N , N ‐dodecyl,methyl‐polyethylenimine (DMPEI). Accompanying viral p24 protein and free viral RNA analysis of solutions exposed to DMPEI‐coated surfaces suggests that virion attachment to the polycationic surface and its subsequent inactivation are the likely mechanism of this phenomenon. Biotechnol. Bioeng. 2013; 110: 2058–2062. © 2013 Wiley Periodicals, Inc.