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An 'omics approach towards CHO cell engineering
Author(s) -
Datta Payel,
Linhardt Robert J.,
Sharfstein Susan T.
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24841
Subject(s) - chinese hamster ovary cell , computational biology , biopharmaceutical , biology , glycomics , metabolic engineering , cell culture , proteomics , microbiology and biotechnology , genetics , gene
Chinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial production of biopharmaceutical products, such as monoclonal antibodies, human growth hormones, cytokines, and blood‐products. Recent advances in recombinant DNA technology have resulted in the bioengineering of CHO cells that have robust gene amplification systems and can also be adapted to grow in suspension cultures. In parallel, recent advances in techniques and tools for decoding the CHO cell genome, transcriptome, proteome, and glycome have led to new areas of study for better understanding the metabolic pathways in CHO cells with the long‐term goal of developing new biologics. This review paper discusses the recent advances in bioengineering strategies in CHO cell lines and the impact of the knowledge gained by CHO cell genomics, transcriptomics, and glycomics on the future of CHO‐cell engineering. Biotechnol. Bioeng. 2013; 110: 1255–1271. © 2013 Wiley Periodicals, Inc.