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Bioreducible poly(amido amine)s with different branching degrees as gene delivery vectors
Author(s) -
Zhang Bo,
Ma Xinpeng,
Murdoch William,
Radosz Maciej,
Shen Youqing
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24772
Subject(s) - transfection , branching (polymer chemistry) , gene delivery , cationic polymerization , amine gas treating , endocytosis , polymer , chemistry , cytotoxicity , biophysics , nanoparticle , polymer chemistry , combinatorial chemistry , organic chemistry , in vitro , nanotechnology , biochemistry , materials science , cell , gene , biology
Based on the knowledge that cationic polymers with different topographical structures behave differently in gene transfection process, herein, we synthesized three biodegradable poly(amido amine)s (PAAs) with the same repeating units and molecular weights except for degree of branching: linear PAA (LPAA), low‐branched PAA (LBPAA), and high‐branched PAA (HBPAA). We found that LBPAA could more effectively compact pDNA into positively charged nanoparticles than both HBPAA and LPAA. LBPAA polyplexes had the highest transfection efficiency among the three PAA polyplexes, and the difference in transfection efficiency is mainly attributed to the endocytosis rate. The cytotoxicity of PAAs was negligible at the transfection doses, probably due to the degradable disulfide bonds. Therefore, we could use branching as a parameter to simply tune a polymer's cellular uptake behavior and transfection efficiency. Biotechnol. Bioeng. 2013; 110: 990–998. © 2012 Wiley Periodicals, Inc.