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Anaerobic ammonia oxidation (ANAMMOX) for side‐stream treatment of anaerobic digester filtrate process performance and microbiology
Author(s) -
Kotay Shireen M.,
Mansell Bryan L.,
Hogsett Mitch,
Pei Huang,
Goel Ramesh
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24767
Subject(s) - anammox , effluent , sequencing batch reactor , pulp and paper industry , ammonia , bioreactor , wastewater , chemistry , nitrosomonas , sewage treatment , anaerobic exercise , continuous stirred tank reactor , nitrification , environmental science , waste management , environmental engineering , biology , denitrification , nitrogen , biochemistry , engineering , physiology , denitrifying bacteria , organic chemistry
A laboratory scale semi‐batch fed anaerobic ammonia oxidation (ANAMMOX) reactor was operated in the lab under two different feeding operations. In the first scenario, termed as phase I, the reactor was seeded and operated with NO 2 ‐N added externally with the filtrate to the reactor in the ratio needed for the successful ANAMMOX. A second reactor was also initiated shortly after the start‐up of the ANAMMOX to accomplish partial nitrification (nitritation reactor) to generate NO 2 ‐N. In phase II, the operation of the ANAMMOX reactor was switched to the mode in which case the partially nitrified effluent from the nitritation reactor was fed to the ANAMMOX reactor. In both phases, real filtrate from a local wastewater treatment plant was used as the feed. The ANAMMOX reactor sustained a loading rate (average 0.33 ± 0.03 with a max of 0.4 g N (L day) −1 ) which is comparable with many other fed‐batch reactors in the literature. Consistent total N removal (average of 82 ± 4%) could be sustained in the ANAMMOX reactor during both phases. The nitritation reactor also consistently enabled a NO 2 ‐N to NH 3 ‐N ratio of 1.2:1 which was needed for the successful operation of the ANAMMOX reactor in phase II. Sequence analysis and FISH showed that Kuenenia stuttgartiensis dominated the enriched ANAMMOX community along with several unidentified, but seemingly enriched, potential ANAMMOX strains. Microbial ecology analysis for nitritation reactor showed the dominance of Nitrosomonas europaea . In summary, this manuscript provides important information on the start‐up and operation of anammox reactor with detailed investigation on microbial ecology in this reactor. Biotechnol. Bioeng. 2013; 110: 1180–1192. © 2012 Wiley Periodicals, Inc.