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Development of a Saccharomyces cerevisiae strain for increasing the accumulation of triacylglycerol as a microbial oil feedstock for biodiesel production using glycerol as a substrate
Author(s) -
Yu Kyung Ok,
Jung Ju,
Ramzi Ahmad Bazli,
Choe Se Hoon,
Kim Seung Wook,
Park Chulhwan,
Han Sung Ok
Publication year - 2013
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24623
Subject(s) - glycerol , diacylglycerol kinase , biodiesel production , strain (injury) , biochemistry , biodiesel , food science , metabolic engineering , chemistry , phospholipid , substrate (aquarium) , raw material , glycerol kinase , saccharomyces cerevisiae , acyltransferase , overproduction , biology , yeast , enzyme , organic chemistry , catalysis , protein kinase c , ecology , membrane , anatomy
Triacylglycerol (TAG) is a microbial oil feedstock for biodiesel production that uses an inexpensive substrate, such as glycerol. Here, we demonstrated the overproduction of TAG from glycerol in engineered Saccharomyces cerevisiae via the glycerol‐3‐phosphate (G3P) pathway by overexpressing the major TAG synthesis. The G3P accumulation was increased 2.4‐fold with the increased glycerol utilization gained by the overexpression of glycerol kinase ( GUT1 ). By overexpressing diacylglycerol acyltransferase ( DGA1 ) and phospholipid diacylglycerol acyltransferase ( LRO1 ), the engineered YPH499 (p GutDgaLro1 ) strain produced 23.0 mg/L lipids, whereas the YPH499 (pESC‐TRP) strain produced 6.2 mg/L total lipids and showed a lipid content that was increased 1.4‐fold compared with 3.6% for the wild‐type strain after 96 h of cultivation. After 96 h of cultivation using glycerol, the overall content of TAG in the engineered strain, YPH499 (p GutDgaLro1 ), yielded 8.2% TAG, representing a 2.3‐fold improvement, compared with 3.6% for the wild‐type strain. The results should allow a reduction of costs and a more sustainable production of biodiesel. Biotechnol. Bioeng. 2013; 110: 343–347. © 2012 Wiley Periodicals, Inc.