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Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC
Author(s) -
Jung Ju,
Yu Kyung Ok,
Ramzi Ahmad Bazli,
Choe Se Hoon,
Kim Seung Wook,
Han Sung Ok
Publication year - 2012
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24524
Subject(s) - surfactin , bacillus subtilis , chemistry , operon , microbiology and biotechnology , biochemistry , lipopeptide , escherichia coli , bacteria , biology , gene , genetics
Surfactin is a biological surfactant with numerous potential applications. In this study, Bacillus subtilis was engineered to improve surfactin production by the activation of two competence‐stimulating pheromones, ComX and competence and sporulation factor (CSF) to stimulate the transcription of srfA operon. Both signaling factors, encoded by comX and phrC , were successfully overexpressed and subsequently increased surfactin production. Surfactin produced by engineered strains showed functional groups similar to the commercially available surfactin analyzed via Fourier transform infrared spectroscopy (FTIR). Surfactin production in the B. subtilis (pHT43‐ comXphrC ) strain was 6.4‐fold greater than in the wild strain, with approximately 135.1 mg/L surfactin produced after 48 h cultivation. To reduce the production costs of surfactin, synthetic wastewater was used, from which the B. subtilis (pHT43‐ comXphrC ) strain produced approximately 140.2 mg/L surfactin. The results obtained demonstrated the production of surfactin from synthetic wastewater, which is beneficial in lowering the overall production costs. Biotechnol. Bioeng. 2012;109: 2349–2356. © 2012 Wiley Periodicals, Inc.