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Transient recombinant protein expression in a human amniocyte cell line: The CAP‐T® cell system
Author(s) -
Fischer Simon,
Charara Nadine,
Gerber Andrea,
Wölfel Jens,
Schiedner Gudrun,
Voedisch Bernd,
Geisse Sabine
Publication year - 2012
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24514
Subject(s) - recombinant dna , microbiology and biotechnology , cell culture , biology , hek 293 cells , gene expression , gene , transient (computer programming) , chinese hamster ovary cell , computational biology , cell , secretion , cell type , genetics , biochemistry , computer science , operating system
The impact of transient gene expression approaches (TGE) on the rapid production of recombinant proteins is undisputed, despite that all efforts are currently relying on two host cell families only, namely HEK293 derivatives and CHO cell line(s). Yet, the increasing complexity of biological targets calls for more than two host cell types to meet the challenges of difficult‐to‐express proteins. For this reason, we evaluated the more recently established novel CAP‐T® cell line derived from human amniocytes for its performance and potential in transient gene expression. Upon careful analyses and adaptation of all process parameters we show here that indeed the CAP‐T® cells are extremely amenable to transient gene expression and recombinant protein production. Additionally, they possess inherent capabilities to express and secrete complex and difficult target molecules, thus adding an attractive alternative to the repertoire of existing host cell lines used in transient production processes. Biotechnol. Bioeng. 2012;109: 2250–2261. © 2012 Wiley Periodicals, Inc.