Premium
Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates
Author(s) -
Fernand Francois,
Rubinsky Liel,
Golberg Alex,
Rubinsky Boris
Publication year - 2012
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24479
Subject(s) - electroporation , curvilinear coordinates , electric field , irreversible electroporation , computer science , biological system , interpolation (computer graphics) , field (mathematics) , cartesian coordinate system , biochemical engineering , chemistry , physics , mathematics , engineering , artificial intelligence , biology , geometry , biochemistry , motion (physics) , quantum mechanics , pure mathematics , gene
The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system. Biotechnol. Bioeng. 2012; 109:2168–2171. © 2012 Wiley Periodicals, Inc.