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Cytochrome c dissolved in 1‐allyl‐3‐methylimidazolium chloride type ionic liquid undergoes a quasi‐reversible redox reaction up to 140°C
Author(s) -
Tamura Kaori,
Nakamura Nobuhumi,
Ohno Hiroyuki
Publication year - 2012
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.24357
Subject(s) - ionic liquid , chemistry , redox , cytochrome , solubility , chloride , cytochrome c , polarizability , hemeprotein , inorganic chemistry , ionic bonding , heme , organic chemistry , catalysis , ion , enzyme , molecule , biochemistry , mitochondrion
Solubility of cytochrome c, a typical heme protein, in a various ionic liquids has been analyzed. The solubility has been discussed with polarity parameters of the ionic liquids. Both hydrogen bond basicity and dipolarity/polarizability of the ionic liquids were confirmed to be influential factors to control the solubilization of cytochrome c. Polar ionic liquids such as 1‐butyl‐3‐methylimidazolium chloride and 1‐allyl‐3‐methylimidazolium chloride solubilized cytochrome c at 80°C, and the dissolved cytochrome c was found to keep its redox activity in these ionic liquids. The redox response of the dissolved cytochrome c was detected in 1‐allyl‐3‐methylimidazolium chloride up to 140°C. Biotechnol. Bioeng. 2012; 109:729–735. © 2011 Wiley Periodicals, Inc.