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A mechanistic model for enzymatic saccharification of cellulose using continuous distribution kinetics II: Cooperative enzyme action, solution kinetics, and product inhibition
Author(s) -
Griggs Andrew J.,
Stickel Jonathan J.,
Lischeske James J.
Publication year - 2012
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23354
Subject(s) - product inhibition , cellobiose , chemistry , cellulose , enzymatic hydrolysis , kinetics , hydrolysis , cellulosic ethanol , substrate (aquarium) , enzyme , enzyme kinetics , product distribution , organic chemistry , cellulase , non competitive inhibition , catalysis , active site , physics , oceanography , quantum mechanics , geology
The projected cost for the enzymatic hydrolysis of cellulosic biomass continues to be a barrier for the commercial production of liquid transportation fuels from renewable feedstocks. Predictive models for the kinetics of the enzymatic reactions will enable an improved understanding of current limitations, such as the slow‐down of the overall conversion rate, and may point the way for more efficient utilization of the enzymes in order to achieve higher conversion yields. A mechanistically based kinetic model for the enzymatic hydrolysis of cellulose was recently reported in Griggs et al. (2011) (Part I). In this article (Part II), the enzyme system is expanded to include solution‐phase kinetics, particularly cellobiose‐to‐glucose conversion by β‐glucosidase (βG), and novel adsorption and product inhibition schemes have been incorporated, based on current structural knowledge of the component enzymes. Model results show cases of cooperative and non‐cooperative hydrolysis for an enzyme system consisting of EG I and CBH I . The model is used to explore various potential rate‐limiting phenomena, such as substrate accessibility, product inhibition, sterically hindered enzyme adsorption, and the molecular weight of the cellulose substrate. Biotechnol. Bioeng. 2012; 109:676–685. © 2011 Wiley Periodicals, Inc.