Premium
Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase
Author(s) -
Yu Kyung Ok,
Jung Ju,
Kim Seung Wook,
Park Chul Hwan,
Han Sung Ok
Publication year - 2012
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23311
Subject(s) - glycerol , biochemistry , saccharomyces cerevisiae , ethanol , chemistry , heterologous , substrate (aquarium) , metabolic engineering , heterologous expression , ethanol fuel , yeast , enzyme , biology , recombinant dna , gene , ecology
The high price of petroleum‐based diesel fuel has led to the development of alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerevisiae that utilize glycerol as substrate, heterologous expression of an unspecific acyltransferase from Acinetobacter baylyi with glycerol utilizing genes was established. As a result, the engineered YPH499 (p GcyaDak , p GupWs‐DgaTCas ) strain produced 0.24 g/L FAEEs using endogenous ethanol produced from glycerol. And this study also demonstrated the possibility of increasing FAEE production by enhancing ethanol production by minimizing the synthesis of glycerol. The overall FAEE production in strain YPH499 fps1Δ gpd2Δ (p GcyaDak , p GupWs‐DgaTCas ) was 2.1‐fold more than in YPH499 (p GcyaDak , p GupWs‐DgaTCas ), with approximately 0.52 g/L FAEEs produced, while nearly 17 g/L of glycerol was consumed. These results clearly indicated that FAEEs were synthesized in engineered S. cerevisiae by esterifying exogenous fatty acids with endogenously produced ethanol from glycerol. This microbial system acts as a platform in applying metabolic engineering that allows the production of FAEEs from cheap and abundant substrates specifically glycerol through the use of endogenous bioethanol. Biotechnol. Bioeng. 2012;109: 110–115. © 2011 Wiley Periodicals, Inc.