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Construction of a recombinant Trichoderma reesei strain expressing Aspergillus aculeatus β‐glucosidase 1 for efficient biomass conversion
Author(s) -
Nakazawa Hikaru,
Kawai Tetsushi,
Ida Noriko,
Shida Yosuke,
Kobayashi Yoshinori,
Okada Hirofumi,
Tani Shuji,
Sumitani Junichi,
Kawaguchi Takashi,
Morikawa Yasushi,
Ogasawara Wataru
Publication year - 2012
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23296
Subject(s) - trichoderma reesei , cellobiose , xylanase , xylan , chemistry , food science , strain (injury) , biomass (ecology) , beta glucosidase , cellulase , recombinant dna , cellulosic ethanol , hydrolysis , cellulose , biochemistry , enzyme , biology , agronomy , gene , anatomy
To develop a Trichoderma reesei strain appropriate for the saccharification of pretreated cellulosic biomass, a recombinant T . reesei strain, X3AB1, was constructed that expressed an Aspergillus aculeatus β‐glucosidase 1 with high specific activity under the control of the xyn3 promoter. The culture supernatant from T . reesei X3AB1 grown on 1% Avicel as a carbon source had 63‐ and 25‐fold higher β‐glucosidase activity against cellobiose compared to that of the parent strain PC‐3‐7 and that of the T . reesei recombinant strain expressing an endogenous β‐glucosidase I, respectively. Further, the xylanase activity was 30% lower than that of PC‐3‐7 due to the absence of xyn3 . X3AB1 grown on 1% Avicel‐0.5% xylan medium produced 2.3‐ and 3.3‐fold more xylanase and β‐xylosidase, respectively, than X3AB1 grown on 1% Avicel. The supernatant from X3AB1 grown on Avicel and xylan saccharified NaOH‐pretreated rice straw efficiently at a low enzyme dose, indicating that the strain has good potential for use in cellulosic biomass conversion processes. Biotechnol. Bioeng. 2012;109: 92–99. © 2011 Wiley Periodicals, Inc.