Premium
Comparative metabolic capabilities for Micrococcus luteus NCTC 2665, the “Fleming” strain, and actinobacteria
Author(s) -
Rokem J. Stefan,
Vongsangnak Wanwipa,
Nielsen Jens
Publication year - 2011
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23212
Subject(s) - micrococcus luteus , actinobacteria , in silico , biology , metabolic network , organism , genome , strain (injury) , gene , genetics , computational biology , escherichia coli , 16s ribosomal rna , anatomy
Putative gene predictions of the Gram positive actinobacteria Micrococcus luteus (NCTC 2665, “Fleming strain”) was used to construct a genome scale reconstruction of the metabolic network for this organism. The metabolic network comprises 586 reactions and 551 metabolites, and accounts for 21% of the genes in the genome. The reconstruction was based on the annotated genome and available biochemical information. M. luteus has one of the smallest genomes of actinobacteria with a circular chromosome of 2,501,097 base pairs and a GC content of 73%. The metabolic pathways required for biomass production in silico were determined based on earlier models of actinobacteria. The in silico network is used for metabolic comparison of M. luteus with other actinomycetes, and hence provides useful information for possible future biotechnological exploitation of this organism, e.g., for production of biofuels. Biotechnol. Bioeng. 2011;108: 2770–2775. © 2011 Wiley Periodicals, Inc.