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Intracellular peptide delivery using amphiphilic lipid‐based formulations
Author(s) -
Weiss Amélie,
Neuberg Patrick,
Philippot Stéphanie,
Erbacher Patrick,
Weill Claire O.
Publication year - 2011
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23182
Subject(s) - intracellular , amphiphile , cytosol , peptide , cytoplasm , chemistry , biochemistry , microbiology and biotechnology , computational biology , biophysics , biology , enzyme , organic chemistry , copolymer , polymer
Abstract Peptides, highly diverse by their nature, are important biochemical and pharmaceutical tools: ligands for cellular receptors, transcription factors, immunosuppressants, vaccines, etc. As the majority of their targets are intracellular, peptides need to cross the plasma membrane and gain access to the cytoplasm. However, due to their physicochemical properties, most peptides need to be entrapped by a molecular vehicle to be able to reach the cytosol compartment. In this study, we present new biological tools to enhance intracellular peptides delivery. Based on electrostatic interactions, two complementary types of amphiphilic molecules have been designed as delivery vehicles. A diverse set of fluorescently labeled peptides have successfully been delivered. This opens the avenue for the use of peptides combined to delivery vehicles as therapeutic aids. Biotechnol. Bioeng. 2011;108: 2477–2487. © 2011 Wiley Periodicals, Inc.