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Metabolic engineering of monoterpene synthesis in yeast
Author(s) -
Fischer Marc J. C.,
Meyer Sophie,
Claudel Patricia,
Bergdoll Marc,
Karst Francis
Publication year - 2011
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23129
Subject(s) - metabolic engineering , escherichia coli , terpenoid , yeast , geraniol , biochemistry , saccharomyces cerevisiae , heterologous expression , farnesyl diphosphate synthase , monoterpene , biology , heterologous , context (archaeology) , biosynthesis , gene , chemistry , botany , recombinant dna , essential oil , paleontology
Abstract Terpenoids are one of the largest and most diverse families of natural compounds. They are heavily used in industry, and the trend is toward engineering modified microorganisms that produce high levels of specific terpenoids. Most studies have focused on creating specific heterologous pathways for sesquiterpenes in Escherichia coli or yeast. We subjected the Saccharomyces cerevisiae ERG20 gene (encoding farnesyl diphosphate synthase) to a set of amino acid mutations in the catalytic site at position K197. Mutated strains have been shown to exhibit various growth rate, sterol amount, and monoterpenol‐producing capacities. These results are discussed in the context of the potential use of these mutated strains for heterologous expression of monoterpenoid synthases, which was investigated using Ocimum basilicum geraniol synthase. The results obtained with up to 5 mg/L geraniol suggest a major improvement compared with previous available expression systems like Escherichia coli or yeast strains with an unmodified ERG20 gene that respectively delivered amounts in the 10 and 500 µg/L range or even a previously characterized K197E mutation that delivered amounts in the 1 mg/L range. Biotechnol. Bioeng. 2011; 108:1883–1892. © 2011 Wiley Periodicals, Inc.