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A highly efficient ADH‐coupled NADH‐recycling system for the asymmetric bioreduction of carbon‐carbon double bonds using enoate reductases
Author(s) -
Tauber Katharina,
Hall Melanie,
Kroutil Wolfgang,
Fabian Walter M.F.,
Faber Kurt,
Glueck Silvia M.
Publication year - 2011
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23078
Subject(s) - chemistry , carbon fibers , reinforced carbon–carbon , double bond , organic chemistry , materials science , composite number , composite material
The asymmetric bioreduction of activated alkenes catalyzed by flavin‐dependent enoate reductases from the OYE‐family represents a powerful method for the production of optically active compounds. For its preparative‐scale application, efficient and economic NADH‐recycling is crucial. A novel enzyme‐coupled NADH‐recycling system is proposed based on the concurrent oxidation of a sacrificial sec ‐alcohol catalyzed by an alcohol dehydrogenase (ADH‐A). Due to the highly favorable position of the equilibrium of ene‐reduction versus alcohol‐oxidation, the cosubstrate is only required in slight excess. Biotechnol. Bioeng. 2011; 108:1462–1467. © 2011 Wiley Periodicals, Inc.