Premium
Consolidated bioprocessing (CBP) performance of Clostridium phytofermentans on AFEX‐treated corn stover for ethanol production
Author(s) -
Jin Mingjie,
Balan Venkatesh,
Gunawan Christa,
Dale Bruce E.
Publication year - 2011
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.23059
Subject(s) - corn stover , cellulosic ethanol , bioprocess , chemistry , fermentation , glucan , food science , biofuel , hydrolysis , ethanol fuel , ethanol fermentation , cellulose , biochemistry , microbiology and biotechnology , biology , paleontology
Consolidated bioprocessing (CBP) is believed to be a potentially cost‐efficient and commercially viable way to produce cellulosic biofuels. In this study, we have evaluated the performance of the CBP organism Clostridium phytofermentans (ATCC 700394) on AFEX‐treated corn stover (AFEX‐CS). Fermentation conditions including temperature, inoculation size, nutrients, and initial pH were investigated. At optimal conditions with 0.5% (w/w) glucan loading of AFEX‐CS, C. phytofermentans hydrolyzed 76% of glucan and 88.6% of xylan in 10 days. These values reached 87% and 102% of those obtained by simultaneous saccharification and co‐fermentation (SSCF) using commercial enzymes and S. cerevisiae 424A. Ethanol titer for CBP was found to be 2.8 g/L which was 71.8% of that yielded by SSCF (3.9 g/L). Decomposition products from AFEX‐CS helped to increase ethanol yield somewhat during CBP. Particle size played a crucial role in the enhancement of sugar conversion by CBP. Biotechnol. Bioeng. 2011; 108:1290–1297. © 2011 Wiley Periodicals, Inc.