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Micropatterns of cell adhesive proteins with poly(ethylene oxide)‐ block ‐Poly(4‐vinylpyridine) diblock copolymer
Author(s) -
Racine J.,
Cheradame H.,
Chu Y.S.,
Thiery J.P.,
Rodriguez I.
Publication year - 2011
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22997
Subject(s) - adhesive , ethylene oxide , copolymer , materials science , polymer , poly ethylene , cell , oxide , polymer chemistry , chemical engineering , nanotechnology , ethylene , chemistry , composite material , organic chemistry , biochemistry , layer (electronics) , catalysis , engineering , metallurgy
Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(ethylene oxide‐ block ‐poly(4‐vinylpyridine) (PEO‐ b ‐P4VP) as a non‐adhesive background to construct micropatterns of cell adhesive proteins. In the method presented, PEO‐ b ‐P4VP is used for its antifouling properties and at the same time, as a photosensitive material to define the micropatterns. The irradiation of PEO‐ b ‐P4VP with a short wavelength UV light through photolithographic mask, causes the polymer to crosslink and immobilize in the areas exposed. In the areas non‐exposed the polymer can be removed. These areas can be subsequent back filled with the adhesive protein of interest to produce the final micropatterned cell chips. Biotechnol. Bioeng. 2011; 108:983–987. © 2010 Wiley Periodicals, Inc.