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Recombinant human cytochrome P450 monooxygenases for drug metabolite synthesis
Author(s) -
Schroer Kirsten,
Kittelmann Matthias,
Lütz Stephan
Publication year - 2010
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22775
Subject(s) - monooxygenase , cytochrome p450 , metabolite , drug metabolism , microsome , xenobiotic , cytochrome , biochemistry , recombinant dna , gene isoform , biology , unspecific monooxygenase , cyp2b6 , enzyme , chemistry , cyp3a4 , gene
Cytochrome P450 monooxygenases (CYPs) are important enzymes in the metabolism of xenobiotics. Therefore, several approaches to clone and overexpress the human isoforms have been made. In addition to microsomes or S9 preparations, these recombinant human isoforms have found diverse application in drug development. We discuss and give examples of the use of bacterial whole cell systems with rec. human CYPs for the preparative scale synthesis of drug metabolites. Biotechnol. Bioeng. 2010;106: 699–706. © 2010 Wiley Periodicals, Inc.