Broad‐host‐range vectors for protein expression across gram negative hosts

Expression of recombinant proteins across a range of different host organisms is often necessary in metabolic engineering applications. Doing so can be facilitated by the use of vectors having origins of replication with a broad‐host‐range, the option for antibiotic resistance cassettes that are compatible with a particular host, in addition to sequences allowing for the effective transcription and translation of target proteins. We have created a modular set of broad‐host‐range expression vectors for protein expression in Gram negative bacteria. These vectors use the broad‐host‐range pBBR1 replicon as well as the arabinose‐inducible P BAD promoter and are available with six different antibiotic resistance cassettes. We have demonstrated the use of these vectors in Escherichia coli , Pseudomonas putida , and Burkholderia cepacia . Biotechnol. Bioeng. 2010;106: 326–332. © 2010 Wiley Periodicals, Inc.