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Broad‐host‐range vectors for protein expression across gram negative hosts
Author(s) -
Prior Jamie E.,
Lynch Michael D.,
Gill Ryan T.
Publication year - 2010
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22695
Subject(s) - biology , replicon , pseudomonas putida , escherichia coli , host (biology) , bacteria , computational biology , burkholderia , vector (molecular biology) , expression vector , genetics , recombinant dna , microbiology and biotechnology , plasmid , dna , gene
Expression of recombinant proteins across a range of different host organisms is often necessary in metabolic engineering applications. Doing so can be facilitated by the use of vectors having origins of replication with a broad‐host‐range, the option for antibiotic resistance cassettes that are compatible with a particular host, in addition to sequences allowing for the effective transcription and translation of target proteins. We have created a modular set of broad‐host‐range expression vectors for protein expression in Gram negative bacteria. These vectors use the broad‐host‐range pBBR1 replicon as well as the arabinose‐inducible P BAD promoter and are available with six different antibiotic resistance cassettes. We have demonstrated the use of these vectors in Escherichia coli , Pseudomonas putida , and Burkholderia cepacia . Biotechnol. Bioeng. 2010;106: 326–332. © 2010 Wiley Periodicals, Inc.