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Influence of the hydrostatic pressure and pH on the asymmetric 2‐hydroxyketone formation catalyzed by Pseudomonas putida benzoylformate decarboxylase and variants thereof
Author(s) -
Berheide Marco,
Peper Stephanie,
Kara Selin,
Long Wei Sing,
Schenkel Steffen,
Pohl Martina,
Niemeyer Bernd,
Liese Andreas
Publication year - 2010
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22650
Subject(s) - pseudomonas putida , chemistry , biocatalysis , hydrostatic pressure , enantiomer , catalysis , stereochemistry , active site , acetaldehyde , saturated mutagenesis , enzyme , organic chemistry , biochemistry , reaction mechanism , thermodynamics , physics , gene , ethanol , mutant
Benzoylformate decarboxylase (BFD) from Pseudomonas putida is a thiamine diphosphate‐dependent (ThDP) enzyme that catalyzes the asymmetric CC bond formation to ( S )‐2‐hydroxypropiophenone [( S )‐HPP] starting from benzaldehyde and acetaldehyde. The enantioselectivity of BFD was shown to be a function of temperature and substrate concentration. It can additionally be changed by site‐directed mutagenesis on hot spot positions in the active site. In this article, we present the effect of hydrostatic pressure up to 250 MPa on the enantioselectivity for the recombinant wtBFD as well as for the variants BFD F464I, BFD A460I, and BFD A460I‐F464I. A general tendency toward lower amounts of ( S )‐HPP could be observed at increasing pressures. For two of these variants an increase in pressure even caused an inversion in the enantioselectivity and thus increasing enantiomeric excesses, respectively. A pressure‐induced increase in enantioselectivity could therefore be observed for the first time in biocatalysis to the best of our knowledge. Furthermore, the pH is shown to be a parameter that also significantly influences the enantioselectivity of the reaction mentioned above. Biotechnol. Bioeng. 2010; 106: 18–26. © 2009 Wiley Periodicals, Inc.